- Gene Roddenberry talking about Star Trek's difficult start and why Star Trek had to "entertain or go off the air."
- Story editor D. C. Fontana on shaping some of the best-loved episodes and her part in establishing the enduring character of Mr. Spock.
- Why William Shatner wanted Star Trek to "have more humor and be more fun."
- Leonard Nimoy discussing how Spock emerged from being a co-star to a breakout iconic character.
- DeForest Kelley on how the fractious relationship between Doctor McCoy and Mr. Spock was developed.
- Nichelle Nichols on the overwhelming reaction to Uhura around the world.
- Guest star William Campbell on his role in "The Squire of Gothos" and the dangers of the Star Trek set!
- The unusual circumstances that led to a first time writer scripting the award-winning episode, "The Tholian Web".
- George Takei on how Leonard Nimoy stood up for his crewmates during the making of Star Trek: The Animated Series and why Sulu is a pioneering character.
- Majel Barrett Roddenberry on the secret ingredient that has made Star Trek endure.
- William Shatner on the struggle to make Star Trek: The Motion Picture.